4J1G
Leanyer orthobunyavirus nucleoprotein-ssRNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.839, 73.832, 79.884 |
| Unit cell angles | 69.74, 89.66, 75.44 |
Refinement procedure
| Resolution | 48.047 - 2.789 |
| R-factor | 0.2367 |
| Rwork | 0.233 |
| R-free | 0.27400 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.445 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | X2DF |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.047 | 2.880 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Number of reflections | 23912 | |
| Completeness [%] | 93.8 | 69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.1-0.2 mM ammonium citrate tribasic (pH 7.0) and 15 - 18% (w/v) PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
