4J0X
Structure of Rrp9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9998 |
Spacegroup name | P 1 |
Unit cell lengths | 48.445, 78.557, 80.029 |
Unit cell angles | 61.77, 86.44, 71.99 |
Refinement procedure
Resolution | 19.963 - 2.502 |
R-factor | 0.2199 |
Rwork | 0.218 |
R-free | 0.26160 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.934 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.088 | 0.319 |
Number of reflections | 32300 | |
<I/σ(I)> | 9 | 1.7 |
Completeness [%] | 95.8 | 85.1 |
Redundancy | 1.9 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 100mM HEPES-K, pH7.5, 25% (v/v) ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 277K |