4J0M

Crystal structure of BRL1 (LRR) in complex with brassinolide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-25
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	105.864, 83.400, 264.064
Unit cell angles	90.00, 97.24, 90.00

Refinement procedure

Resolution	29.829 - 2.500
R-factor	0.1896
Rwork	0.187
R-free	0.23860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rgz
RMSD bond length	0.010
RMSD bond angle	1.524
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	75248
Completeness [%]	94.7	96.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.1	291	0.2M ammonium citrate dibasic, 20% (w/v) polyethylene glycol (PEG) 3350, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 291K