4J0C
tannin acyl hydrolase from Lactobacillus plantarum (native structure)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 |
Unit cell lengths | 46.458, 62.791, 83.803 |
Unit cell angles | 70.39, 86.01, 79.41 |
Refinement procedure
Resolution | 19.677 - 1.650 |
R-factor | 0.1661 |
Rwork | 0.165 |
R-free | 0.19190 |
Structure solution method | MIR |
RMSD bond length | 0.009 |
RMSD bond angle | 1.187 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 1.740 | |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.077 | 0.550 |
Number of reflections | 100941 | |
Completeness [%] | 88.2 | |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 281 | 0.1M tri-sodium citrate pH 5.5, 20% PEG 3000, VAPOR DIFFUSION, SITTING DROP, temperature 281K |