4J01
Crystal Structure of Fischerella Transcription Factor HetR complexed with 29mer DNA target
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.91948 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.157, 182.182, 78.366 |
| Unit cell angles | 90.00, 91.38, 90.00 |
Refinement procedure
| Resolution | 35.904 - 3.246 |
| R-factor | 0.235 |
| Rwork | 0.241 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4izz |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.833 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1161)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.246 | 3.246 |
| Number of reflections | 33964 | |
| <I/σ(I)> | 5.5 | 1.7 |
| Completeness [%] | 98.7 | 91.7 |
| Redundancy | 4.4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 25 % PEG 2000MME, 100 mM Hepes pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
