4IZX
Macrolepiota procera ricin B-like lectin (MPL) in complex with lactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-10 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.800, 57.158, 61.229 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.220 - 1.100 |
R-factor | 0.1086 |
Rwork | 0.107 |
R-free | 0.13210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ion |
RMSD bond length | 0.013 |
RMSD bond angle | 1.558 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 1.120 |
High resolution limit [Å] | 1.100 | 2.980 | 1.100 |
Rmerge | 0.050 | 0.024 | 0.387 |
Number of reflections | 43310 | ||
<I/σ(I)> | 9 | ||
Completeness [%] | 88.2 | 95.5 | 42.9 |
Redundancy | 7 | 6.4 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M Imidazole, 0.5 M Sodium citrate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |