4IZO
Crystal structure of kinase Phosphoribosylaminoimidazole carboxylase, ATPase subunit from Burkholderia thailandensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-01-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.127092 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 53.930, 53.930, 465.920 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.390 - 1.850 |
R-factor | 0.1837 |
Rwork | 0.182 |
R-free | 0.21410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4e4t |
RMSD bond length | 0.014 |
RMSD bond angle | 1.518 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.2) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.900 |
High resolution limit [Å] | 1.850 | 8.270 | 1.850 |
Rmerge | 0.071 | 0.053 | 0.132 |
Number of reflections | 35480 | 512 | 2336 |
<I/σ(I)> | 35.1 | 36.25 | 19.34 |
Completeness [%] | 97.2 | 86.1 | 90.4 |
Redundancy | 18.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | EB JCSG screen a9: 200mM Ammonium chloride, 20% PEG 3350 PEG 1500; ButhA.00036.a.A1.PS01171 at 20 mg/ml, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |