4IZH
Crystal Structure of the Alpha1 dimer of Thermus thermophilus Transhydrogenase in P6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97949 |
Spacegroup name | P 6 |
Unit cell lengths | 162.318, 162.318, 73.883 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 140.610 - 1.800 |
R-factor | 0.16754 |
Rwork | 0.167 |
R-free | 0.18024 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l7d |
RMSD bond length | 0.013 |
RMSD bond angle | 1.525 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 140.610 | 1.890 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.048 | 0.437 |
Number of reflections | 97773 | |
<I/σ(I)> | 11.3 | 1.7 |
Completeness [%] | 99.5 | 97.4 |
Redundancy | 5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 3.5 | 293 | MemStart MemSys kit, condition E2: 0.1M Sodium chloride, 0.1M Na citrate pH 3.5, 0.1M Lithium sulphate, 30% v/v PEG 400, VAPOR DIFFUSION, temperature 293K |