4IZE

Crystal Structure of IL-36gamma

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-08-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	89.315, 89.315, 37.879
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.872 - 2.000
R-factor	0.1802
Rwork	0.178
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.177
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.050
High resolution limit [Å]	2.000	8.950	2.000
Rmerge	0.097	0.028	0.666
Number of reflections	10682	150	740
<I/σ(I)>	26.49	65.78	5.27
Completeness [%]	98.8	97.4	95.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	PEG 5000MME, NaAcetate, pH 4.5, vapor diffusion, hanging drop, temperature 298K