4IY9
Bmlp3 - C2 crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-18 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 154.150, 34.560, 93.140 |
Unit cell angles | 90.00, 97.99, 90.00 |
Refinement procedure
Resolution | 38.100 - 2.100 |
R-factor | 0.2188 |
Rwork | 0.216 |
R-free | 0.27150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4efp |
RMSD bond length | 0.019 |
RMSD bond angle | 1.810 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.164 | 2.184 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.060 | 0.496 |
Number of reflections | 26553 | |
<I/σ(I)> | 15.9 | 2.58 |
Completeness [%] | 91.3 | 86.3 |
Redundancy | 3.7 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 2.0 M (NH4)2SO4, 0.1 M NH4I, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |