4IXL
Crystal structure of endo-beta-1,4-xylanase from the alkaliphilic Bacillus sp. SN5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Detector technology | CCD |
Collection date | 2012-03-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9788 |
Spacegroup name | P 43 |
Unit cell lengths | 59.415, 59.415, 153.736 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.812 - 1.490 |
Rwork | 0.196 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dck |
Data reduction software | APEX |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 1.490 |
Rmerge | 0.027 |
Number of reflections | 85897 |
Completeness [%] | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 0.1 M lithium sulfate monohydrate, 0.1 M ADA pH 6.5, 12% w/v PEG4000, 2% v/v 2-Propanol, VAPOR DIFFUSION, SITTING DROP |