4IXA
Structure of DNA-binding domain of the response regulator SaeR from Staphylococcus epidermidis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2012-10-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.201, 53.784, 111.658 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.164 - 2.150 |
R-factor | 0.2061 |
Rwork | 0.203 |
R-free | 0.26190 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.202 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.031 | 0.152 |
Number of reflections | 11686 | |
Completeness [%] | 99.1 | 99.8 |
Redundancy | 6.8 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 277 | 0.1M Bis-Tris, 23%(w/v) PEG3350, 0.2M MgCl2, pH 5.3, VAPOR DIFFUSION, SITTING DROP, temperature 277K |