4IX3
Crystal structure of a Stt7 homolog from Micromonas algae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97939 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.714, 133.139, 55.046 |
| Unit cell angles | 90.00, 116.29, 90.00 |
Refinement procedure
| Resolution | 33.280 - 1.350 |
| R-factor | 0.13511 |
| Rwork | 0.133 |
| R-free | 0.17392 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.738 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 150593 | |
| <I/σ(I)> | 28.99 | 2.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 100mM HEPES, 200mM MgCl2, 25% PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
