4IWM
Crystal Structure of the Conserved Hypothetical Protein MJ0927 from Methanocaldococcus jannaschii (in P21 form)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2013-01-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97921, 0.97938, 0.96414 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 95.370, 77.136, 131.737 |
Unit cell angles | 90.00, 105.36, 90.00 |
Refinement procedure
Resolution | 26.901 - 2.700 |
R-factor | 0.1921 |
Rwork | 0.189 |
R-free | 0.25770 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.136 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.450 | |
Number of reflections | 51206 | |
<I/σ(I)> | 2.8 | |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 0.1M (NH4)2SO4, 0.3M sodium formate, 0.1M sodium acetate, 3% PGA-LM, 20% MPD, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |