4IW7
Crystal structure of 8-amino-7-oxononanoate synthase (bioF) from Francisella tularensis.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | H 3 2 |
Unit cell lengths | 113.486, 113.486, 158.128 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.770 - 2.250 |
R-factor | 0.18828 |
Rwork | 0.186 |
R-free | 0.23836 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g6w |
RMSD bond length | 0.014 |
RMSD bond angle | 1.536 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.290 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.079 | 0.742 |
Number of reflections | 18866 | |
<I/σ(I)> | 29.7 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.9 | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.7 M Sodium citrate tribasic dihydrate, 0.1 M Tris pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |