4IW1
HSA-fructose complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2011-12-10 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.363, 87.985, 59.350 |
Unit cell angles | 90.00, 102.70, 90.00 |
Refinement procedure
Resolution | 48.410 - 2.560 |
R-factor | 0.24341 |
Rwork | 0.239 |
R-free | 0.31537 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ao6 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.692 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.560 |
Number of reflections | 18509 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | 25%-30% PEG 3350, 50mM potassium phosphate, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |