4IVZ
A Y37F mutant of C.Esp1396I bound to its highest affinity operator site OM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.540, 146.700, 47.790 |
| Unit cell angles | 90.00, 93.21, 90.00 |
Refinement procedure
| Resolution | 34.630 - 3.100 |
| R-factor | 0.17194 |
| Rwork | 0.170 |
| R-free | 0.21478 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ufd |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.708 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.1.26) |
| Phasing software | PHASER (2.5.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 146.810 | 3.310 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.111 | 0.666 |
| Number of reflections | 11810 | |
| <I/σ(I)> | 12.7 | 1.8 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 0.1 M SPG buffer, 25 % w/v PEG 1500, 10 uM Spermidine , pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
