4IVE
Crystal structure of a polyadenylate-binding protein 3 (PABPC3) from Homo sapiens at 2.30 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979493,0.918401,0.979284 |
Spacegroup name | P 43 |
Unit cell lengths | 67.395, 67.395, 101.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.128 - 2.300 |
R-factor | 0.2011 |
Rwork | 0.201 |
R-free | 0.21210 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | XSCALE (March 15, 2012) |
Phasing software | SHELX |
Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.128 | 43.128 | 2.380 |
High resolution limit [Å] | 2.300 | 4.930 | 2.300 |
Rmerge | 0.097 | 0.053 | 0.756 |
Number of reflections | 20106 | 2103 | 1919 |
<I/σ(I)> | 12.03 | 25 | 2.4 |
Completeness [%] | 99.5 | 99.7 | 97.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.62 | 277 | 26.00% polyethylene glycol 6000, 0.1M MES pH 5.62, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |