4IU9

Crystal structure of a membrane transporter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.07082
Spacegroup name	P 21 21 21
Unit cell lengths	117.439, 118.211, 127.056
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.840 - 3.005
R-factor	0.2638
Rwork	0.261
R-free	0.31630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4iu8
RMSD bond length	0.009
RMSD bond angle	1.283
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.110
High resolution limit [Å]	3.000	3.000
Number of reflections	30017
Completeness [%]	83.9	62.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	100 mM Tris-HCl (pH 8.5), 33% (w/v) PEG 400 and 100 mM KCl, VAPOR DIFFUSION, HANGING DROP, temperature 291K