4ITQ
Crystal structure of hypothetical protein SCO1480 bound to DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-31 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 43.420, 71.782, 102.925 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.439 - 2.700 |
R-factor | 0.2672 |
Rwork | 0.262 |
R-free | 0.31500 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.789 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
Rmerge | 0.055 | 0.026 | 0.539 |
Number of reflections | 5202 | ||
<I/σ(I)> | 31.2 | 66.4 | 1.4 |
Completeness [%] | 99.6 | 100 | 94.7 |
Redundancy | 6.1 | 6.5 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 277 | 19% PEG 3350, 210 mM KSCN, 5% ethylene glycol, 100 mM HEPES pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |