4ITK
The structure of C.reinhardtii Ferredoxin 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-31 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 25.429, 26.458, 31.016 |
| Unit cell angles | 102.56, 104.35, 100.30 |
Refinement procedure
| Resolution | 25.000 - 1.180 |
| R-factor | 0.11049 |
| Rwork | 0.109 |
| R-free | 0.14745 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rfk |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.497 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | MrBump/Molrep |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.280 |
| High resolution limit [Å] | 1.180 | 1.180 |
| Number of reflections | 24232 | |
| <I/σ(I)> | 2.59 | |
| Completeness [%] | 99.4 | 98.1 |
| Redundancy | 6.06 | 2.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 0.1 M HEPES pH 7.0, 3.2 M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
