4IT4
Crystal structure of residues 1-211 of CG17282
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.098, 90.193, 249.671 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.650 - 2.500 |
R-factor | 0.1987 |
Rwork | 0.197 |
R-free | 0.23030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Residues 1-120 of CG17282 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.962 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
Rmerge | 0.129 | 0.095 | 0.695 |
Number of reflections | 53197 | ||
<I/σ(I)> | 12.7 | ||
Completeness [%] | 99.9 | 99.4 | 99.7 |
Redundancy | 7.5 | 7.8 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 8% (v/v) Tacsimate pH 4.5, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |