4ISB
Crystal Structure of Apo Mtb FadD10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2007-02-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.318, 107.913, 85.689 |
| Unit cell angles | 90.00, 106.92, 90.00 |
Refinement procedure
| Resolution | 48.886 - 2.200 |
| R-factor | 0.2288 |
| Rwork | 0.227 |
| R-free | 0.27280 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.255 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | RESOLVE (2.15) |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
| Rmerge | 0.120 | 0.059 | 0.606 |
| Number of reflections | 50192 | ||
| <I/σ(I)> | 9.9 | ||
| Completeness [%] | 99.4 | 98.3 | 99.1 |
| Redundancy | 3.6 | 3.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG 6000, LiSO4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
