4IS7
Crystal Structure of the CASKIN2 SAM Domain Tandem
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-09 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.00 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 96.460, 96.460, 119.170 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.509 - 2.751 |
| R-factor | 0.2506 |
| Rwork | 0.248 |
| R-free | 0.26660 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.680 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.509 | 2.923 |
| High resolution limit [Å] | 2.751 | 2.751 |
| Number of reflections | 9004 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 2.4 M NaF, 15% glycerol, 0.1 M Tris-HCl, 0.5 mM protein, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
