4IS4
The glutamine synthetase from the dicotyledonous plant M. truncatula is a decamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 99.335, 101.672, 188.084 |
| Unit cell angles | 90.00, 103.69, 90.00 |
Refinement procedure
| Resolution | 96.511 - 2.350 |
| R-factor | 0.1767 |
| Rwork | 0.175 |
| R-free | 0.21660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d3a |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.165 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.000 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.077 | 0.338 |
| Number of reflections | 149655 | |
| <I/σ(I)> | 19.2 | 4 |
| Completeness [%] | 99.0 | 96.8 |
| Redundancy | 6.3 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 287 | 0.12 M triammonium citrate, 9.2% PEG3350, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 287K |
