4IRX
Crystal structure of Caulobacter myo-inositol binding protein bound to myo-inositol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-27 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9794 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 83.121, 34.958, 181.830 |
Unit cell angles | 90.00, 102.60, 90.00 |
Refinement procedure
Resolution | 19.900 - 1.451 |
R-factor | 0.1659 |
Rwork | 0.165 |
R-free | 0.18940 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.316 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.8.1_1168) |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.900 |
High resolution limit [Å] | 1.450 |
Rmerge | 0.080 |
Number of reflections | 77441 |
<I/σ(I)> | 14.8 |
Completeness [%] | 99.0 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 292 | 75 mN HEPES pH7.4, PEG3350 25%, 5 mM myo-inositol, VAPOR DIFFUSION, HANGING DROP, temperature 292K |