4IRW

Co-crystallization of streptavidin-biotin complex with a lanthanide-ligand complex gives rise to a novel crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	93.15
Detector technology	IMAGE PLATE
Collection date	2011-08-13
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5417
Spacegroup name	C 2 2 2
Unit cell lengths	64.423, 71.395, 55.494
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.859 - 1.396
R-factor	0.1256
Rwork	0.123
R-free	0.15360
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.431
Data reduction software	d*TREK (9.9.8.9 W9RSSI)
Data scaling software	d*TREK
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.860	27.860	1.450
High resolution limit [Å]	1.396	3.010	1.396
Rmerge	0.066	0.054	0.377
Total number of observations		18962	1215
Number of reflections	21044
<I/σ(I)>	15.4	41.2	1.8
Completeness [%]	81.5	99	22.9
Redundancy	6.26	6.76	2.08

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	1.5 uL protein (pH 8.0) incubated with saturated biotin solution + 1.5 uL 200 mM Na3[Tb(Dpa)3] + 3 uL 60% v/v MPD, VAPOR DIFFUSION, HANGING DROP, temperature 298K