4IRA
CobR in complex with FAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.1170 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 103.193, 103.193, 133.103 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.377 - 2.200 |
| R-factor | 0.22485 |
| Rwork | 0.223 |
| R-free | 0.25032 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.173 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 53.377 | 74.120 | 2.320 |
| High resolution limit [Å] | 2.200 | 6.960 | 2.200 |
| Number of reflections | 21883 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.2 M (NH4)2SO4, 0.02 M Na Acetate , pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
