4IQS
RNA 8mer duplex modified with 4-Se-Uridine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2009 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 47.006, 47.006, 354.105 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.730 - 2.600 |
| R-factor | 0.19686 |
| Rwork | 0.194 |
| R-free | 0.25824 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.846 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.053 | 0.460 |
| Number of reflections | 7113 | |
| <I/σ(I)> | 35.9 | 2 |
| Completeness [%] | 80.0 | 95.8 |
| Redundancy | 10 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 10% MPD, 40 mM Na Cacodylate pH 6.0, 12 mM Spermine tetra-HCl, 12 mM NaCl/80 mM KCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
