4IQA
Crystal Structure Analysis of the E228L Mutant of Human CLIC1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2009-10-05 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.768, 71.938, 83.074 |
Unit cell angles | 90.00, 90.44, 90.00 |
Refinement procedure
Resolution | 36.120 - 2.490 |
R-factor | 0.2485 |
Rwork | 0.245 |
R-free | 0.30370 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.399 |
Data reduction software | SAINT (V7.68A) |
Data scaling software | SAINT (V7.68A) |
Phasing software | PHASER (2.5.1) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 36.120 | 5.355 | 2.575 |
High resolution limit [Å] | 2.486 | 4.251 | 2.486 |
Number of reflections | 17255 | ||
<I/σ(I)> | 19.841 | ||
Completeness [%] | 99.0 | ||
Redundancy | 11.16 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2M NaCl, 0.1 M HEPES 25% (w/v) PEG 3350, 0.02% azide, 5 mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |