4IQ8
Crystal structure of glyceraldehyde-3-phosphate dehydrogenase 3 from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-06-11 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.9792 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 116.129, 116.129, 119.179 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.490 |
R-factor | 0.2167 |
Rwork | 0.214 |
R-free | 0.27358 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3pym |
RMSD bond length | 0.010 |
RMSD bond angle | 1.182 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.530 |
High resolution limit [Å] | 2.490 | 2.490 |
Rmerge | 0.459 | |
Number of reflections | 14571 | |
<I/σ(I)> | 7.71 | |
Completeness [%] | 99.7 | 100 |
Redundancy | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 287 | 12% PEG 3350, 0.1M sodium malonate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 287K |