4IQ0
Crystal structure of oxidoreductase, Gfo/Idh/MocA family from Streptococcus pneumoniae with reductive methylated Lysine
Replaces: 2HO3Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97940 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.915, 152.386, 100.237 |
Unit cell angles | 90.00, 103.12, 90.00 |
Refinement procedure
Resolution | 38.600 - 2.000 |
R-factor | 0.1481 |
Rwork | 0.145 |
R-free | 0.19520 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.111 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.074 | 0.170 |
Number of reflections | 90011 | |
<I/σ(I)> | 25.3 | 7 |
Completeness [%] | 99.7 | 98.6 |
Redundancy | 3.7 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M Lithium sulfate, 0.1 M TRIS, 1.26 M Ammonium sulfate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |