4IPY
HIV capsid C-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Collection date | 2012-09-28 |
Wavelength(s) | 0.9725 |
Spacegroup name | P 1 |
Unit cell lengths | 40.141, 43.578, 55.189 |
Unit cell angles | 74.02, 74.21, 69.77 |
Refinement procedure
Resolution | 19.700 - 1.640 |
R-factor | 0.1779 |
Rwork | 0.176 |
R-free | 0.21800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a8o |
RMSD bond length | 0.006 |
RMSD bond angle | 1.074 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: dev_1261)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.700 |
High resolution limit [Å] | 1.640 |
Rmerge | 0.046 |
Number of reflections | 40722 |
<I/σ(I)> | 22.6 |
Completeness [%] | 95.3 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | reservoir contained 0.2 M magnesium formate dihydrate (pH 7.0) and 20% (w/v) polyethylene glycol 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |