4IPV
Crystal structure of Pseudomonas aeruginosa (strain: PAO1) type IV minor pilin FimU in space group P65
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | P 65 |
Unit cell lengths | 40.687, 40.687, 272.922 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.236 - 1.400 |
R-factor | 0.1655 |
Rwork | 0.164 |
R-free | 0.19590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ipu |
RMSD bond length | 0.005 |
RMSD bond angle | 1.022 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.044 | 0.354 |
Number of reflections | 49829 | |
<I/σ(I)> | 53.8 | 5.9 |
Completeness [%] | 99.4 | 93.9 |
Redundancy | 15.4 | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.2 M sodium chloride, 0.1 M Tris-HCl, 25% (w/v) PEG 3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |