4IOV
The structure of AAVrh32.33, a Novel Gene Delivery Vector
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9125 |
| Spacegroup name | P 1 |
| Unit cell lengths | 246.396, 247.521, 250.332 |
| Unit cell angles | 70.38, 65.37, 60.17 |
Refinement procedure
| Resolution | 40.000 - 3.500 |
| R-factor | 0.271 |
| Rwork | 0.271 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.630 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 397503 | |
| <I/σ(I)> | 2.98 | 1.65 |
| Completeness [%] | 68.5 | 66 |
| Redundancy | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.3 mM KCl, 0.7 mM KH2PO4, 8.2 mM NaCl, 4 mM Na2HPO4, 5% glycerol, and 3.5% polyethylene glycol 8000 (PEG 8000) , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
