4IOS
Structure of phage TP901-1 RBP (ORF49) in complex with nanobody 11.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-11-18 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.931 |
Spacegroup name | H 3 |
Unit cell lengths | 148.280, 148.280, 104.590 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.010 - 2.400 |
R-factor | 0.2019 |
Rwork | 0.201 |
R-free | 0.22690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hem |
RMSD bond length | 0.009 |
RMSD bond angle | 1.210 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.086 | 0.534 |
Number of reflections | 33444 | |
<I/σ(I)> | 15.5 | 2.25 |
Completeness [%] | 99.8 | 97.7 |
Redundancy | 6.3 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 20% PEG 1000, 0.2M Lithium Sulfate, 0.1M Na Phosphate Citrate, pH 4.2 , VAPOR DIFFUSION, SITTING DROP, temperature 293K |