4IOK
N10-formyltetrahydrofolate synthetase from Moorella thermoacetica with ADP, XPO
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Temperature [K] | 100 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 91.170, 212.976, 53.444 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 106.490 - 2.497 |
R-factor | 0.17349 |
Rwork | 0.164 |
R-free | 0.25876 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3RBO |
RMSD bond length | 0.011 |
RMSD bond angle | 1.616 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 106.490 | 2.590 |
High resolution limit [Å] | 2.497 | 2.497 |
Rmerge | 0.085 | |
Number of reflections | 37221 | |
Completeness [%] | 91.5 | 64.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 38 to 46% (w/v) saturated ammonium sulfate (AS), 1 mM DTT, and 1 to 3.5% (w/v) PEG 1000 or PEG 1450, in 50 - 75 mM KMB (pH 7.0 - 8.0), VAPOR DIFFUSION, HANGING DROP, temperature 298K |