4IOJ
N10-formyltetrahydrofolate synthetase from Moorella thermoacetica with sulfate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Temperature [K] | 100 |
Spacegroup name | H 3 2 |
Unit cell lengths | 161.203, 161.203, 256.915 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 122.670 - 2.202 |
R-factor | 0.18093 |
Rwork | 0.178 |
R-free | 0.23302 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3PZX |
RMSD bond length | 0.018 |
RMSD bond angle | 1.935 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 122.670 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.076 |
Number of reflections | 64918 |
<I/σ(I)> | 10.3 |
Completeness [%] | 99.9 |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 38 to 46% (w/v) saturated ammonium sulfate (AS), 1 mM DTT, and 1 to 3.5% (w/v) PEG 1000 or PEG 1450, in 50-75 mM KMB (pH 7.0 - 8.0), VAPOR DIFFUSION, HANGING DROP, temperature 298K |