4IO3
Crystal Structure of the AvGluR1 ligand binding domain complex with aspartate at 1.66 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.132, 100.364, 56.896 |
| Unit cell angles | 90.00, 116.40, 90.00 |
Refinement procedure
| Resolution | 35.760 - 1.660 |
| R-factor | 0.157 |
| Rwork | 0.157 |
| R-free | 0.18000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4io2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.268 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: dev_1039)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.690 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.055 | 0.580 |
| Number of reflections | 65127 | |
| <I/σ(I)> | 22.3 | 2.1 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 293 | 22.5% PEG 3350, 0.1 M NaCitrate, 0.1 M BisTris Propane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
