4INW
Structure of Pheromone-binding protein 1 in complex with (11Z,13Z)-hexadecadienal
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-12 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 65 |
Unit cell lengths | 57.455, 57.455, 93.268 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.720 - 1.140 |
R-factor | 0.161 |
Rwork | 0.160 |
R-free | 0.18300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.022 |
RMSD bond angle | 1.802 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | PHASER (1.3.1) |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 28.720 |
High resolution limit [Å] | 1.140 |
Completeness [%] | 97.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 295 | Purified AtraPBP1 was crystallized at room temperature by the hanging drop vapor diffusion method. Drops composed of 1ul protein solution (30 mg/ml) and 1ul of the precipitant solution were suspended over a reservoir containing the precipitant solution (1.6 M sodium citrate pH 6.5). Crystals used in data collection were transferred into Paratone-N oil and flash-cooled in a stream of liquid nitrogen at 110 K, VAPOR DIFFUSION, HANGING DROP, temperature 295K |