4INR
Yeast 20S proteasome in complex with the vinyl sulfone LU102
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-11-04 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 136.320, 301.050, 144.320 |
Unit cell angles | 90.00, 113.13, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.700 |
R-factor | 0.22433 |
Rwork | 0.224 |
R-free | 0.23073 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ryp |
RMSD bond length | 0.004 |
RMSD bond angle | 0.808 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.049 | 0.473 |
Number of reflections | 287367 | |
<I/σ(I)> | 17.98 | 2.52 |
Completeness [%] | 98.4 | 90 |
Redundancy | 3.4 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 20 mM magnesium acetate, 0.1 M MES, 12% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |