4INN
Protein HP1028 from the human pathogen Helicobacter pylori belongs to the lipocalin family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.240, 79.150, 131.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.670 - 2.600 |
| R-factor | 0.19384 |
| Rwork | 0.191 |
| R-free | 0.24773 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.662 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.710 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.108 | 0.225 |
| Number of reflections | 11665 | |
| <I/σ(I)> | 19 | 9.5 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 13.4 | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 0.2 M lithium sulfate, 0.1 M Tris-HCl, 30% PEG4000, pH 8.5, VAPOR DIFFUSION, temperature 298K |
