4INA
Crystal Structure of the Q7MSS8_WOLSU protein from Wolinella succinogenes. Northeast Structural Genomics Consortium Target WsR35
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97923 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.639, 95.490, 104.427 |
Unit cell angles | 90.00, 101.80, 90.00 |
Refinement procedure
Resolution | 45.061 - 2.488 |
R-factor | 0.195 |
Rwork | 0.193 |
R-free | 0.22200 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.251 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AutoSol |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.600 |
High resolution limit [Å] | 2.490 | 2.490 |
Rmerge | 0.087 | 0.474 |
Number of reflections | 57476 | |
<I/σ(I)> | 10.6 | 2.6 |
Completeness [%] | 96.9 | 97.7 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch crystallization under oil | 6.5 | 277 | 20% PEG MME 5000, 0.1M BIS-TRIS, pH 6.5, Microbatch crystallization under oil, temperature 277K |