4IN0
Crystal Structure of human splicing factor dim2/TXNL4B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2005-12-05 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.325, 63.640, 51.148 |
Unit cell angles | 90.00, 92.29, 90.00 |
Refinement procedure
Resolution | 30.567 - 1.327 |
R-factor | 0.1303 |
Rwork | 0.128 |
R-free | 0.18490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xbs |
RMSD bond length | 0.022 |
RMSD bond angle | 2.189 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.567 |
High resolution limit [Å] | 1.327 |
Number of reflections | 56401 |
Completeness [%] | 97.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 18% PEG8000, 0.2 M magnesium acetate tetrahydate, 0.1 M sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |