4IMK

Uncrossed Fab binding to human Angiopoietin 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	65.969, 86.670, 205.813
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.761 - 2.202
R-factor	0.2003
Rwork	0.199
R-free	0.22570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.824
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	46.761
High resolution limit [Å]	2.200
Number of reflections	60561

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	15%(w/v) PEG4000, 0.1 M Tri-Na-citrate, 15%(v/v) isopropanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K