4IM6
LRR domain from human NLRP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-20 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0000 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 84.690, 84.690, 130.470 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.800 - 1.650 |
R-factor | 0.13691 |
Rwork | 0.134 |
R-free | 0.18253 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1z7x |
RMSD bond length | 0.023 |
RMSD bond angle | 2.271 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.800 | 1.700 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.038 | 0.376 |
Number of reflections | 33885 | |
<I/σ(I)> | 51.6 | 9.71 |
Completeness [%] | 99.8 | 100 |
Redundancy | 19.8 | 17.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1 M Tris pH 8.0, 0.02 M MgCl2, 25 % (w/v) PAA 5100, VAPOR DIFFUSION, HANGING DROP, temperature 293K |