4IM3
Structure of Tank-Binding Kinase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 141.320, 141.320, 85.421 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.796 - 3.342 |
| R-factor | 0.2226 |
| Rwork | 0.220 |
| R-free | 0.27850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.213 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.630 |
| High resolution limit [Å] | 3.300 | 5.240 | 3.300 |
| Number of reflections | 13368 | ||
| Completeness [%] | 87.3 | 98.1 | 51.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 4% MPD, 100 mM Hepes pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
