4ILV
Structure of the dioxygenase domain of SACTE_2871, a novel dioxygenase carbohydrate-binding protein fusion from the cellulolytic bacterium Streptomyces sp. SirexAA-E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 62.463, 62.463, 195.997 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.807 - 2.060 |
R-factor | 0.1859 |
Rwork | 0.183 |
R-free | 0.23710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.094 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 2.100 |
High resolution limit [Å] | 2.060 | 5.590 | 2.060 |
Rmerge | 0.076 | 0.031 | 0.736 |
Number of reflections | 24673 | ||
<I/σ(I)> | 7.3 | ||
Completeness [%] | 98.3 | 98.1 | 95.7 |
Redundancy | 9.5 | 8.4 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | Protein Solution (10 mg/ml protein, 0.05 M NaCl, 0.010 M MOPS pH 7.0) mixed in a 1:1 ratio with the Well Solution (16% Polyethylene glycol 3350, 200mM Sodium Malonate, 100mM Bis-Tris pH 5.5). Cryoprotected with 16% Polyethylene glycol 3350, 200mM Sodium Malonate, 15% ethylene glycol, 100mM Bis-Tris pH 5.5, vapor diffusion, hanging drop, temperature 298K |