4ILJ
Crystal structure of an Prp8p RNaseH W1911A mutant protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-02 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.600, 85.022, 95.955 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.800 - 2.000 |
R-factor | 0.1822 |
Rwork | 0.180 |
R-free | 0.22620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eo9 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.188 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.060 | 0.670 |
Number of reflections | 42335 | |
<I/σ(I)> | 17.9 | 2.6 |
Completeness [%] | 99.7 | 99.6 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.1 M Tris-Cl pH 8.5, 12% PEG 3350, 100 mM NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 298K |