4ILH

Crystal structure of an Aar2p C-terminal deletion mutant in conplex with Prp8p RNaseH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-17
Detector	RAYONIX MX-225
Wavelength(s)	0.9184
Spacegroup name	C 1 2 1
Unit cell lengths	79.890, 76.760, 91.860
Unit cell angles	90.00, 105.88, 90.00

Refinement procedure

Resolution	33.953 - 1.850
R-factor	0.1703
Rwork	0.168
R-free	0.21740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sbt
RMSD bond length	0.009
RMSD bond angle	1.157
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	1.900
High resolution limit [Å]	1.850	1.850
Rmerge	0.080	0.880
Number of reflections	45500
<I/σ(I)>	16.1	2
Completeness [%]	99.7	100
Redundancy	3.4	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	0.1 M Tris-HCl, pH 8.0, 19 % (w/v) PEG 4000 and 100 mM KCl, VAPOR DIFFUSION, SITTING DROP, temperature 277K